موضوعات مقالات

دانلود مقالات » علوم سلامت و پزشکی » داروسازی

Development and characterization of a replicon-based phenotypic assay for assessing HCV NS4B from clinical isolates

Development and characterization of a replicon-based phenotypic assay for assessing HCV NS4B from clinical isolates
Antiviral Research
نام ژورنال: Antiviral Research
نویسنده یا نویسندگان مقاله: Sonal Rajyaguru, Huiling Yang, Ross Martin, Michael D. Miller, Hongmei Mo
زبان: انگلیسی
سال انتشار یا تایید مقاله: 2013
شماره صفحات: 328-336
فرمت فایل: PDF
حجم فایل: 706 کیلوبایت
شناسه DOI:
قیمت: رایگان
   
دانلود مقاله



Cell-penetrating peptide (CPP)-mediated intracellular drug delivery system, often specifically termed as “the Trojan horse approach”, has become the “holy grail” in achieving effective delivery of macromolecular compounds such as proteins, DNA, siRNAs, and drug carriers. It is characterized by the unique cell- (or receptor-), temperature-, and payload-independent mechanisms, therefore offering potent means to improve poor cellular uptake of a variety of macromolecular drugs. Nevertheless, this “Trojan horse” approach also acts like a double-edged sword, causing serious safety and toxicity concerns to normal tissues or organs for in vivo application, due to lack of target selectivity of the powerful cell penetrating activity. To overcome this problem of potent yet non-selective penetration vs. targeting delivery, a number of “smart” strategies have been developed in recent years, including controllable CPP-based drug delivery systems based on various stimuli-responsive mechanisms. This review article provides a fundamental understanding of these smart systems, as well as a discussion of their real-time in vivo applicability.